Independent Scientific Validation of The Fountain of Youth Lifestyle Tripeptide Collagen

The Fountain of Youth Lifestyle (FoYL) Tripeptide Collagen has been independently tested by a leading European university using pharmaceutical laboratory models.

In-Vitro & In-Situ Studies

FoYL's tripeptide collagen has undergone independent, third-party, pharmaceutical laboratory testing by the University of Studies of Milan to evaluate not just composition, but functional biological performance - something never done before for collagen ingredients.

The independent studies were conducted by:
Università degli Studi di Milano (University of Studies of Milan) - Dipartimento di Scienze Farmaceutiche (Department of Pharmaceutical Sciences) (DiSFaRM)
Final Scientific Report Date: 27 June 2025

Click Here to view a synopsis of the clinical trial results (opens in a new window).

What These Studies Confirm - And Why It Matters

Independent laboratory testing demonstrated that FoYL's tripeptide collagen is fundamentally different from standard or hydrolysed collagen products.

Rather than acting solely as a source of amino acids, FoYL's ultra-low molecular weight tripeptide collagen was shown to behave as a bioactive peptide system, interacting with key metabolic and vascular pathways under controlled laboratory conditions.

Key Outcomes from the Independent Testing

Up to 5× Higher Predicted Bioactivity

Advanced peptide mapping showed that FoYL's tripeptide collagen exhibits up to five times greater predicted biological activity compared with conventional collagen peptides.

What this means for you:
More of the collagen you consume is biologically "active", not just digested and discarded.

Precision Peptides for Superior Absorption

300-500 Dalton molecular weight
Over 80% tripeptides

This size range aligns with the optimal zone for collagen peptide absorption and signalling, allowing peptides to be rapidly absorbed while retaining their functional activity.

What this means for you:
Your body can absorb and use this collagen more efficiently than standard, larger collagen peptides.

Supports Natural Metabolic Signalling

Laboratory testing showed that FoYL's tripeptide collagen:

Stimulated pathways involved in natural satiety signalling
Helped maintain balanced post-meal metabolic responses
Demonstrated activity at realistic daily intake levels

What this means for you:
Support for metabolic balance - not through stimulants or drugs, but by working with your body's own signalling systems.

Supports Healthy Circulation Pathways

The studies also demonstrated interaction with enzyme systems involved in:

Vascular relaxation
Blood flow regulation

What this means for you:
FoYL collagen supports whole-body wellbeing, not just skin, hair, or joints.

Proven to Remain Active in the Gut

Importantly, FoYL's tripeptide collagen retained biological activity in human intestinal cell models (in-situ testing).

Most collagen products are never tested this way or to this level.

What this means for you:
This collagen doesn't just look good on paper - it remains active where absorption actually occurs.

Gentle & Well Tolerated

High cell viability observed in testing
Confirms compatibility under laboratory conditions

What this means for you:
Designed for daily use as part of a long-term wellness routine.

Why This Sets FoYL Collagen Apart

Most collagen products rely on:

Generic hydrolysed collagen
Amino-acid profiles
Broad, non-specific claims

FoYL's collagen is supported by independent university-led testing demonstrating:

Higher bioactivity
Better absorption potential
Functional metabolic and vascular relevance

This positions FoYL collagen as a next-generation, precision-designed collagen, not a commodity protein powder.

The Fountain of Youth Lifestyle Tripeptide Collagen.
The Next Evolution of Collagen.

Reference

Independent scientific evaluation conducted by: Università degli Studi di Milano (University of Studies of Milan) - Dipartimento di Scienze Farmaceutiche (Department of Pharmaceutical Sciences) (DiSFaRM)
Final report dated 27.06.2025

Additional Scientific Studies

Please Note: Not all related to Tripeptide Collagen

#	First author, year (journal)	Key finding (dose / model)	Free full-text / DOI
1	Inoue 2016 (J Sci Food Agric)	5 g day⁻¹ "NGI" bovine HC for 8 weeks: ↑ skin hydration (+7-12%), elasticity (+4-9%); ↓ wrinkle depth (−8%) in 56 women (DB-RCT).	PubMed: https://pubmed.ncbi.nlm.nih.gov/26840887
2	Ohara 2007 (J Agric Food Chem)	Human crossover study (5 men): plasma peak 20-60 nmol mL⁻¹ Hyp-di/tripeptides 1-2 h after 10 g bovine HC.	https://pubmed.ncbi.nlm.nih.gov/17253720
3	Iwai 2005 (J Agric Food Chem)	First identification of food-derived Pro-Hyp in human blood after 10 g porcine/bovine HC; evidence of intact peptide absorption.	https://pubmed.ncbi.nlm.nih.gov/16076145
4	Ichikawa 2010 (Int J Food Sci Nutr)	9 g day⁻¹ bovine HC for 4 weeks: plasma Pro-Hyp 4× ↑; confirms dose-dependence of tripeptide appearance.	https://pubmed.ncbi.nlm.nih.gov/19961355
5	Watanabe-Kamiyama 2010 (J Agric Food Chem)	Rats given low-MW bovine HC (average 3 kDa): ↑ femoral bone mineral content (+7%).	https://pubmed.ncbi.nlm.nih.gov/19957932
6	Yamamoto 2016 (Biol Pharm Bull)	Human study comparing purified CTP-50 vs generic HC: plasma & urinary Pro-Hyp ↑ proportional to Gly-Pro-Hyp intake.	https://pubmed.ncbi.nlm.nih.gov/26934933
7	Proksch 2014 - VERISOL® (Skin Pharmacol Physiol)	2.5 g bovine-hide BCP for 8 weeks: ↓ eye-wrinkle volume (−20%); procollagen I +65%.	https://pubmed.ncbi.nlm.nih.gov/24401291
8	Proksch 2013 (Skin Pharmacol Physiol)	2.5 or 5 g bovine HC for 8 weeks: ↑ skin elasticity 15%; effect persisted 4 weeks post-intervention.	https://pubmed.ncbi.nlm.nih.gov/23949208
9	Song 2013 (Clin Interv Aging)	3 g collagen tripeptide for 12 weeks: ↑ skin hydration (+28%) & elasticity (+19%) in healthy adults.	https://pubmed.ncbi.nlm.nih.gov/24131075
10	Hexsel 2017 (J Cosmet Dermatol)	2.5 g bovine BCP × 24 weeks: ↑ nail growth rate (+12%) & ↓ fragility (−42%).	https://pubmed.ncbi.nlm.nih.gov/28786550

Summaries

#	Study & model	What they measured	Direction of the main result
1	Inoue 2016 - 8-week DB-RCT, 56 women, 5 g/day bovine HC	Corneometer skin hydration, Cutometer elasticity, fringe-projection wrinkle depth	↑ Hydration (+7-12%), ↑ elasticity (+4-9%), ↓ wrinkle depth (−8%) vs placebo
2	Ohara 2007 - single-dose crossover PK, 5 men, 10 g bovine HC	Plasma levels of Hyp-di/tri-peptides	Peaks of 20-60 nmol mL⁻¹ Pro-Hyp / Hyp-Gly at 1-2 h post-dose (none at baseline)
3	Iwai 2005 - single-dose PK, 5 volunteers, 10 g porcine/bovine HC	Identification of intact tripeptides in serum	First evidence of Pro-Hyp appearance in human blood after oral collagen
4	Ichikawa 2010 - dose-escalation PK, 4 volunteers	Dose-response of plasma Hyp-peptides (30-385 mg kg⁻¹)	Clear, linear ↑ in peptide C_max with higher doses (6 → 33 nmol mL⁻¹)
5	Watanabe-Kamiyama 2010 - ovariectomised-rat model, 4 wks LMW bovine HC	Femoral bone organic content & BMC	↑ Bone organic matter and BMC (+7%) vs controls
6	Yamamoto 2016 - human crossover, 9 subjects, tripeptide-enriched CTP vs generic HC	Plasma & urinary Gly-Pro-Hyp / Pro-Hyp	Higher and longer-lasting peptide levels with CTP-50; magnitude proportional to tripeptide dose
7	Proksch 2014 - 114 women, 2.5 g VERISOL® for 8 wks	3-D stereo wrinkle volume & dermal biopsies	−20% eye-wrinkle volume; +65% procollagen-I vs placebo (p < 0.05)
8	Proksch 2013 - 69 women, 2.5 / 5 g collagen peptides for 8 wks	Cutometer elasticity (R2)	+15% elasticity at both doses; effect persisted 4 wks after stopping
9	Song 2013 (Kim BJ) - 64 adults, 3 g collagen tripeptide, 12 wks	Hydration & elasticity vs control	+28% hydration, +19% elasticity; both significant vs control
10	Hexsel 2017 - 25 women, 2.5 g VERISOL®, 24 wks (open-label)	Nail growth rate & brittleness	+12% growth rate, −42% cracked/brittle nails; 64% "overall improvement" by physician assessment

Independently Tested by the University of Milan

Most collagen never gets absorbed. Ours is made to be.

Most collagen pieces are simply too large, so your body breaks them down like ordinary food protein. Ours is small enough to be absorbed and put to work - and in 2025, the University of Milan independently tested it and found it up to 5x more active than standard collagen.

See The Proof ↓ Shop The Collagen

Made for Australians who want collagen their body can actually use.

Fountain of Youth Lifestyle Tripeptide Collagen

The Difference Comes Down To Size

Four numbers tell the story.

300-500

Daltons

Small enough for your body to absorb, instead of breaking it down as food.

>80%

Active Collagen

More than 80% is the active form - the part that actually does the work.

More Active

Up to five times more active than standard collagen in the University's laboratory testing.

University of Milan

Independently Tested - 2025

Tested by university scientists, not by us. That independence is the point.

Side By Side

How ours compares with ordinary collagen.

The Standard Option

Ordinary Hydrolysed Collagen

Pieces of many different sizes
Most pieces too large to absorb well
Less of the active form
Structure varies from batch to batch

The Fountain of Youth Lifestyle

Pure Tripeptide Collagen

Consistently small, active pieces
Sized to be absorbed, not discarded
More than 80% active collagen
Composition independently analysed

How It Works

Three simple steps, from your morning drink to your body.

1. It gets absorbed

Because the pieces are so small, they pass through the gut wall and into the bloodstream far more easily than ordinary collagen.

2. More of it arrives

More than 80% of every serve is the active form of collagen, so a much higher share of what you take actually gets delivered.

3. It gets to work

In the University's laboratory testing, this combination showed up to 5x more activity than standard collagen.

In simple terms: it gets in, more of it arrives, and it does more once it is there.

Laboratory models evaluate biological behaviour under controlled conditions and do not represent a claim of clinical outcomes.

What The Independent Testing Found

They didn't just test what's in it. They tested what it does.

In 2025, pharmaceutical scientists at the University of Milan put our collagen through independent laboratory testing - checking not only its make-up, but how it actually behaves. That level of testing is something most collagen brands never do.

Up to 5x more active

The University found our collagen showed up to five times more biological activity than ordinary collagen.

What this means for you

More of what you take each day is put to use, not just digested and discarded.

Sized to be absorbed

At 300 to 500 Daltons, the pieces sit in the ideal size range for the body to take in and use.

What this means for you

Your body absorbs and uses this collagen more easily than standard, larger collagen.

Works with your appetite

Testing showed it works with the body's own natural fullness and after-meal responses, at a normal daily serve.

What this means for you

Gentle support for everyday balance - no stimulants, nothing artificial.

Supports healthy circulation

The studies showed interaction with the body's own systems for healthy blood flow.

What this means for you

Support for whole-body wellbeing - not just skin, hair, and joints.

Stays active where it counts

It stayed active in tests using human gut cells - the place where absorption actually happens. Most collagen is never tested to this level.

What this means for you

It keeps working right where your body takes it in.

Gentle enough for every day

Testing confirmed it is gentle and well tolerated.

What this means for you

Made for daily use, as part of a long-term routine.

Independent Testing Conducted By

University of Milan - Department of Pharmaceutical Sciences

Universita degli Studi di Milano - Dipartimento di Scienze Farmaceutiche (DiSFaRM)

Final Scientific Report: 27 June 2025

Read the report synopsis (PDF) →

Why This Matters As We Age

Your body makes less collagen every year. This is how you support it.

What slowing collagen feels like

From our mid-twenties, collagen production gradually declines - and it shows up in the ways many of us know well:

Stiffer joints, especially in the morning
Thinner, drier skin
Less bounce and elasticity
Slower recovery after activity
Changes after menopause

Where collagen support is studied to help

Collagen peptides are studied for supporting the body's own structural pathways as production slows:

Skin firmness and elasticity
Cartilage and joint comfort
Tendon and ligament resilience
Connective tissue recovery
Bone strength support

For The Curious

Want to go deeper? The research is all here.

Every study is one click away - including eleven published papers. Please note: not all relate to tripeptide collagen specifically.

Skin elasticity studies

Clinical studies on collagen peptides have demonstrated improvements in skin elasticity and skin structure over time - linked to collagen's role in supporting the skin's underlying framework.

Joint and mobility studies

Research into collagen peptides has shown potential benefits for joint comfort, mobility, and cartilage health - particularly in older adults and active people.

Bone and structural support

Studies indicate collagen plays a role in supporting bone structure and mineral density, particularly alongside good nutrition and an active lifestyle.

Absorption studies

Research suggests smaller collagen pieces may be more readily absorbed and used by the body - which is exactly why ours is made small.

The full study list (11 published papers)

#	First author, year (journal)	Key finding (dose / model)	Free full-text / DOI
1	Inoue 2016 (J Sci Food Agric)	5 g day⁻¹ "NGI" bovine HC for 8 weeks: ↑ skin hydration (+7-12%), elasticity (+4-9%); ↓ wrinkle depth (−8%) in 56 women (DB-RCT).	PubMed: https://pubmed.ncbi.nlm.nih.gov/26840887
2	Ohara 2007 (J Agric Food Chem)	Human crossover study (5 men): plasma peak 20-60 nmol mL⁻¹ Hyp-di/tripeptides 1-2 h after 10 g bovine HC.	https://pubmed.ncbi.nlm.nih.gov/17253720
3	Iwai 2005 (J Agric Food Chem)	First identification of food-derived Pro-Hyp in human blood after 10 g porcine/bovine HC; evidence of intact peptide absorption.	https://pubmed.ncbi.nlm.nih.gov/16076145
4	Ichikawa 2010 (Int J Food Sci Nutr)	9 g day⁻¹ bovine HC for 4 weeks: plasma Pro-Hyp 4× ↑; confirms dose-dependence of tripeptide appearance.	https://pubmed.ncbi.nlm.nih.gov/19961355
5	Watanabe-Kamiyama 2010 (J Agric Food Chem)	Rats given low-MW bovine HC (average 3 kDa): ↑ femoral bone mineral content (+7%).	https://pubmed.ncbi.nlm.nih.gov/19957932
6	Yamamoto 2016 (Biol Pharm Bull)	Human study comparing purified CTP-50 vs generic HC: plasma & urinary Pro-Hyp ↑ proportional to Gly-Pro-Hyp intake.	https://pubmed.ncbi.nlm.nih.gov/26934933
7	Proksch 2014 - VERISOL® (Skin Pharmacol Physiol)	2.5 g bovine-hide BCP for 8 weeks: ↓ eye-wrinkle volume (−20%); procollagen I +65%.	https://pubmed.ncbi.nlm.nih.gov/24401291
8	Proksch 2013 (Skin Pharmacol Physiol)	2.5 or 5 g bovine HC for 8 weeks: ↑ skin elasticity 15%; effect persisted 4 weeks post-intervention.	https://pubmed.ncbi.nlm.nih.gov/23949208
9	Song 2013 (Clin Interv Aging)	3 g collagen tripeptide for 12 weeks: ↑ skin hydration (+28%) & elasticity (+19%) in healthy adults.	https://pubmed.ncbi.nlm.nih.gov/24131075
10	Hexsel 2017 (J Cosmet Dermatol)	2.5 g bovine BCP × 24 weeks: ↑ nail growth rate (+12%) & ↓ fragility (−42%).	https://pubmed.ncbi.nlm.nih.gov/28786550

Plain-English study summaries

#	Study & model	What they measured	Direction of the main result
1	Inoue 2016 - 8-week DB-RCT, 56 women, 5 g/day bovine HC	Corneometer skin hydration, Cutometer elasticity, fringe-projection wrinkle depth	↑ Hydration (+7-12%), ↑ elasticity (+4-9%), ↓ wrinkle depth (−8%) vs placebo
2	Ohara 2007 - single-dose crossover PK, 5 men, 10 g bovine HC	Plasma levels of Hyp-di/tri-peptides	Peaks of 20-60 nmol mL⁻¹ Pro-Hyp / Hyp-Gly at 1-2 h post-dose (none at baseline)
3	Iwai 2005 - single-dose PK, 5 volunteers, 10 g porcine/bovine HC	Identification of intact tripeptides in serum	First evidence of Pro-Hyp appearance in human blood after oral collagen
4	Ichikawa 2010 - dose-escalation PK, 4 volunteers	Dose-response of plasma Hyp-peptides (30-385 mg kg⁻¹)	Clear, linear ↑ in peptide C_max with higher doses (6 → 33 nmol mL⁻¹)
5	Watanabe-Kamiyama 2010 - ovariectomised-rat model, 4 wks LMW bovine HC	Femoral bone organic content & BMC	↑ Bone organic matter and BMC (+7%) vs controls
6	Yamamoto 2016 - human crossover, 9 subjects, tripeptide-enriched CTP vs generic HC	Plasma & urinary Gly-Pro-Hyp / Pro-Hyp	Higher and longer-lasting peptide levels with CTP-50; magnitude proportional to tripeptide dose
7	Proksch 2014 - 114 women, 2.5 g VERISOL® for 8 wks	3-D stereo wrinkle volume & dermal biopsies	−20% eye-wrinkle volume; +65% procollagen-I vs placebo (p < 0.05)
8	Proksch 2013 - 69 women, 2.5 / 5 g collagen peptides for 8 wks	Cutometer elasticity (R2)	+15% elasticity at both doses; effect persisted 4 wks after stopping
9	Song 2013 (Kim BJ) - 64 adults, 3 g collagen tripeptide, 12 wks	Hydration & elasticity vs control	+28% hydration, +19% elasticity; both significant vs control
10	Hexsel 2017 - 25 women, 2.5 g VERISOL®, 24 wks (open-label)	Nail growth rate & brittleness	+12% growth rate, −42% cracked/brittle nails; 64% "overall improvement" by physician assessment